Validation and robustness testing of an OPLC method for the determination of aflatoxins in wheat. Toxins in wheat.
PAPP, E., FARKAS, A., OTTA, K.H. and MINCSOVICS, E.
JPC – Journal of Planar Chromatography – Modern TLC 13: 328 – 332.
2000
บทคัดย่อ
The validation of an OPLC procedure developed for the determination of AFB1, B2, G1 and G2 in wheat is described. Samples were extracted with acetonitrile – water (9:1 v/v) and the extracts were filtered and evaporated to dryness. OPLC was performed with chloroform – toluene – tetrahydrofuran (15 + 15 + 1 v/v) as mobile phase. Before the separation an OPLC prewashing step was necessary; this eliminated the time – consuming and costly cleanup steps of other chromatographic methods. Recovery was greater than 54% and the relative standard deviation was less than 10%. The limit of detection for AFB1, B2, G1 and G2 was 0.14, 0.15, 0.100 and 0.018 ng, respectively. The robustness of the procedure was also determined and found be critically dependent on the type of plate used (TLC or HPTLC).