Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India.
THIRUMALA – DEVI, K., MAYO, M.A., REDDY, G., EMMANUEL, K.E., LARONDELLE, Y. and REDDY, D.V.R.
Food Additives and Contaminants 18: 830 – 835.
2001
บทคัดย่อ
OA contamianation of black pepper, coriander seeds, powdered ginger and turmeric powder was estimated using indirect competitive ELISA. Samples were extracted with 0.5% KCI in 70% methanol and diluted in phosphate – buffered saline containing 0.05% Tween 20 and 0.2% bovine serum albumin. ELISA results were compared with those made by HPLC. All estimates were within 1 – 2 standard deviations of the ELISA values. More than 90% of OA added to spice samples was recovered from samples containing between 5 and 100 mg/kg. In 126 samples obtained from retail shops, OA was found to exceed 10 mg/kg in 14/26 black pepper samples, 20/50 coriander samples, 2/25 ginger samples and 9/25 turmeric samples. This is the first record in India of the occurrence of OA in what are some of the most widely used spices in Indian cooking.