บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

A modified Rpl3 gene from rice confers tolerance of the Fusarium graminearum mycotoxin deoxynivalenolto transgenic tobacco.

HARRIS, L.J. and GLEDDIE, S.C.

Physiological and Molecular Plant Pathology 58: 173 – 181.

2001

บทคัดย่อ

A modified Rpl3 gene from rice confers tolerance of the Fusarium graminearum mycotoxin deoxynivalenolto transgenic tobacco.

DON is a potent inhibitor of eukaryotic protein synthesis and is believed to play a role in fungal pathogenesis on cereal crops worldwide. The putatiove site of action by DON hs been theorised to be the 60S robosomal protein  L3 (RPL3). A rice (Oryza saliva) cDNA encoding the ribosomal protein RPL3 was modified so that amino acid residue 258 is changed from tryptophan to cysteine, a change which is believed to confer resistance to similar mycotoxins in yeast. Both versions of the rice Rp13 genes were introduced into two species of tobacco by Agrobacterium tumefaciens co-cultivation and expressed under the control of a constitutive promoter. Results indicate a possible mechanism of host plant resistance to the fungal pathogen Fusaruim graminearum among  the susceptible cereal species based on the expression of modified Rp 13 genes.