Trichothecene toxin effects on barley callus and seedling growth.
DANLEEN. L.S. and MCCORMICK, S.P.
Cereal Research Communications 29 : 115 – 120.
2001
บทคัดย่อ
The effects of DON and the related toxin diacetoxyscirpenol (DAS) on barley callus growth and seedling germination, root and shoot growth were investigated. The effects of the toxins on callus growth were small. Although statistically significant differences were seen at the higher toxin levels after eight weeks, the differences were not large enough for selection between toxin resistant and toxin susceptible calli, Neither toxin had an effect on seed germination. DON at 5 and 10 mg/L significantly reduced root growth by day 7 and 10 mg/L significantly reduced shoot growth by day 4. The effects of DAS were greater, with all toxin levels significantly reducting root gowth by day 2 and shoot growth by day 4. DAS may be usable for screening progeny of transgenic plants for expression and segregation of anti – toxin genes.