บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Cultivar – dependent expression of a maize lipoxygenase responsive to seed infesting fungi.

WILSON, R.A., GARDNER, H.W. and KELLER, N.P.

Molecular Plant – Microbe Interactions 14: 980 – 987.

2001

บทคัดย่อ

Cultivar – dependent expression of a maize lipoxygenase responsive to seed infesting fungi.

Fatty acid derivatives of the plant lipoxygenase (LOX) pathway are implicated in the Aspergillus – seed interaction in maize. The 9(S) – hydroperoxide derivative of linoleic acid pronotes transcription of aflatoxin genes, whereas the 13(S) – hydroperoxide derivative decreases aflatoxin gene expression and production: both are sporulation factors. In this study, screening maize LOX expressed sequence tags identified on clone, essap 92, which is highly expressed in Aspergillus – infected seed susceptible to aflatoxin contamination and repressed in lines with resistance to aflatoxin contamination. The accumulation of essap 92 transcript was similar during Fusarium infection. Product – specificity analysis of the CSSAP 92 protein shows that it preferentially adds oxygen to carbon 9 of linoleic acid. Because 9(S) – hydroperoxy linoleic acid has been implicated as an aflatosin – signalling molecule, it is possible that essap 92 could be used as a biomarker that is indicative of aflatoxin resistance in maize lines.