บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Development of a flow-through enzyme immunoassay and application in screening green coffee samples for ochratoxin A with confirmation by high- performance liquid chromatography.

SIBANDA, L., DESAEGER, S., BAUTERS, T.G.M., NELIS, H.J. and VANPETEGHEM, C.

Journal of food Protection 64: 1597 – 1602.

2001

บทคัดย่อ

Development of a flow-through enzyme immunoassay and application in screening green coffee samples for ochratoxin A with confirmation by high- performance liquid chromatography.

A flow through enzyme immunoassay for the screening of green coffee bean for OA has been developed. The test has a sensitivity of 8 mg/kg and calculated recoveries ranged from 70 to 80% and from 86 to 95% for spike and naturally contaminated samples, respectively. Green coffee samples (15 Arabica and 7 Robusta) were analysed for intrinsic fungal contamination confirmed by HPLC. Results of the screening procedure found thai 4/22 samples were contaminated with OA at 8 mg/kg or higher. The HPLC method found that OA level ranged from 27 to 168 mg/kg. A fifth sample, which was shown to be negative during screening, has an OA concentration of 4 mg/kg. There were no false negative or positives recorded and the flow- through enzyme immunoassay results correlated with those obtained by HPLC.