Determination of fumonisins B1 and B2 in corn and corn flakes by liquid chromatography with immunoaffinity column cleanup: collaborative study.
VISCONTI, A., SOLFRIZZO,M. and DEGIRoLAMO, A.
Journal of AOAC International 84: 1828 – 1837.
2001
บทคัดย่อ
An LC method for the determination of FB1 and FB2 in corn and corn flakes was collaboratively studies by 23 laboratories. The method involved double extraction with acetonitrile- methanol – water (25 + 25 + 50), cleanup through an immunoaffinite column and LC determination of the fumonisins after derivatisation with o-phthaldialdehyde. Relative standard deviations for the intralaboratory repeatability (RSDr) of the corn analyses for FB1 and FB2 ranged from 19 to 24 and 19 to 27%, respectively, and for corn flakes from 9 to 21% and 8 to 22%,respectively. Mean recoveries of FB1 and FB2 from spiked corn flakes were 110 and 97%, respectively. HORRAT ratios for corn ranged from 1.44 to 1.53 for FB1 and from 0.96 to 1.48 for FB2 , whereas for corn flakes they ranged from 1.60 to 1.82 and 1.39 to 1.68, respectively.