บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of zearalenone content in cereals and feedstuffs by immunoaffin-Ity column coupled with liquid chromatography.

FAZEKAS, B. and TAR, A.

Journal of AOAC International 84: 1453-1459.

2001

บทคัดย่อ

Determination of zearalenone content in cereals and feedstuffs by immunoaffin-Ity column coupled with liquid chromatography.

The  ZEA  content  of  maize,  wheat,  barley,  swine feed and  poultry feed samples  was  determined by  immunoaffinity  column  cleanup  followed  by  LC  (IAC-LC).  Samples  were  extracted  in  methanol – water  (8 + 2, v/v)  solution.  The  filtered  extract  was  diluted  with distilled  water  and  applied to immunoaffiniry  columns.  ZEA was  eluted  with  methanol,  dried  by  evaporation  and dissolved  in  acetonitrile-water (3 + 7, v/v). ZEA  was  separated by  isocratic elution  of  acetonirile-water  (50 +  50, v/v)  on  reversed  phase  C18  column.  The  quantitative  analysis  was  performed by  fluorescence  detector  and  confirmation  was  based on the UV  spectrum  obtained  by  a  diode  array  detector.  The  mean  recovery  rate  of ZEA  was 82-97%. The  limit of  detection  of  ZEA  by  fluorescence  was  10  mg/kg.  The  specificity of  the  method  was  increased  by  using  fluorescence  detection  in  parallel  with  UV  detection.