ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

A study of the suitability of gas chromatography-electron capture detection for the analysis of deoxynivalenol in cereals.

ESKOLA, M., BOONZAAIJER, G., VAN OSENBRUGGEN, W.A., RIZZO, A. and TIJMENSEN, G.

Mycotoxin Research 16: 73-90.

2000

บทคัดย่อ

A GC-electron  capture  detection (GCECD) method  for  the  analysis  of DON in cereals  was  investigated.  The sample  was  extracted with  a mixture  of  acetonitrile-water  and  purified  with  a MycoSep#225  column.  The  silylation  was  performed with  Tri-Sil-TBT  reagent,  followed by dilution with  hexane and  a  washing step with  buffer. By  using Tri – Sil – TBT  reagent, no double  peaks  were observed for  DON  in the gas  chromato  grams,  in  comparison  with  two  other silylation  reagents, TMSI  and  TriSil-Z.  Trichothecolone  was  used as an  internal  standard for DON  and  1,1 – bix – (4 – chlorophenyl)- 2,2- dichloroethylene  (DDE), was used as a  GC  standard.