Development of a multi-tiered approach to the in vitro prescreening of clay-based enterosorbents.
LEMKE, S.L., OTTINGER, S.E., MAYURA, K., AKE, C.L., PIMPUKDEE, K., WANG, N. and PHILLIPS, T.D.
Animal Feed Science and Technology 93: 17-29.
2001
บทคัดย่อ
Three previously extablished in vitro methods: single- concentration sorption, isotherms and chemisorption index (Calpha) were compared as methods for predicting the adsorption of AFB1 from solution by four sorbents: HSCAS, charcoal, clinoptilolite and sand. In addition, a gastrointestinal (GI) model was utilised to measure adsorption. Finally, maize was included in modified isotherm studies to examine interactions. HSCAS proved efficacious in all testing methods. Binding of AFB, by charcoal was comparable to HSCAS but was hindered in the presence of maize. Under GI conditions, clinoptilolite demonstrated catalytic activity not observed in the other methods. Results indicate theat the GL model is a repid and more physiologically relevant method of screening sorbents.