Determination of aflatoxins B1, B2, G1 and G2 in spices using a multifunctional column clean-up.
AKIYAMA, H., GODA, Y., TANAKA, T. and TOYODA, M.
Journal of Chromatography A 932: 153-157.
2001
บทคัดย่อ
A rapid and simple method using a multifunctional column which contains lipophilic and charged active sites was developed to analyse aflatoxins in various spices. After extraction by acetonitrile – water (9:1) and cleanup using Multi- sep #228 column, the aflatoxins and aflatoxin- TFA derivatives are determined using LC with fluorescence detection. Recoveries of each AFB1, B2, G1 and G2 spiked to red peper, white pepper, black pepper, nutmeg and tear grass at 10 mg/kg were over 80-85% in all instances. The minimum detectable concentration for aflatoxins in red pepper was 0.5 mg/kg.