บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Immunoaffinity column as cleanup tool for a direct competitive enzyme – linked immunosorbent assay of cyclopiazonic acid in corn, peanuts, and mixed feed

YU, W.J., DORNER, J.W. and CHU, F.S.

Journal of AOAC International 81 : 1169 – 1175.

1998

บทคัดย่อ

Immunoaffinity column as cleanup tool for a direct competitive enzyme – linked immunosorbent assay of cyclopiazonic acid in corn, peanuts, and mixed feed.

An immunoaffinity column (JAC) for cyclopiazonic acid (CPA) was prepared by coupling a CPA – specific monoclonal antibody to CNBr activated sepharose 4B. A direct competitive ELISA (dc – ELESA) was used to study the chromatographic behaviour of a 0.2 mL gel column with a binding capacity for CPA of 4mg/column as well as to evaluate its efficacy as a cleanup tool for analysis of naturally coocurring CPA. Recoveries of CPA added to corn, peanut and mixed feed extracts in the range 10 – 200 mg/kg were 88 – 105, 86 – 100 and 90 – 110%< respectively. Detection limits were 2.0, 4.4 and 4.7 mg/kg for corn, mixed feed and peanuts, respectively. Twenty two peanut samples naturally contaminated with CPA were subjected to both IAC and solvent partition cleanup followed by dc – ELISA. Although a good correlation between data obtained from both methods was obtained, the slope of the linear regression was low, indication loss during solvent partition cleanup.