บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

High – performance liquid chromatography atmospheric – pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed.

Rosenberg, E., Krska, R., Wissiack, R., Kmetov, V., Josephs, R., Razzai, E, and Grasserbauer, M.

Journal of Chromatography A 819: 277 – 288.

1998

บทคัดย่อ

High – performance liquid chromatography atmospheric – pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed.

A method is described for thedetermination of ZEA in food and feed, based on HPLC – MS with an atmospheric pressure chemical ionisation (APCI) interface after extraction from cereals and cleanup by either conventional solid phase or immunoaffinity cartridges. The APCI interface parameters were optimised to  provide detection of ZEA with maximum sensitivity after reversed phase separation of ZEA on a C18 column with acetonitrile - water (40: 60, v/v). Using APCI –MS detection, the sensitivity of the method was improved by a factor of about 50 in comparison to  HPLC with fluorescence detection, allowing determination of ZEA down in 0.12 mg/kg maize.