บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

High – performance liquid chromatographic method for the determination of moniliformin in corn.

MUNIMBAZI, C. and BULLERMAN, L.B.

Journal of AOAC International 81: 999 – 1004.

1998

บทคัดย่อ

High – performance liquid chromatographic method for the determination of moniliformin in corn.

An HPLC method using UV absorption was developed for thedetermination of moniliformin in corn. The toxin was extracted with water containing 1% tetrabutylammnonium hydrogen sulphate (w/v), partitioned into dichloromethane, then evaporated to dryness. The residue was dissolved in water and applied to disposable strong – anion exchange solid phase extraction tube. Adsorbed moniliformin was eluted from the tube with 0.05M sodium dihydrogen phospate monohydrate and was determined by ion – pair reversedphase chromatography and UV measurement. The limit of determination was 0.025 mg/kg corn. Recoveries of moniliformin from corn spiked at 0.025, 0.05, 0.25 ad 1.0 mg/kg averaged 97, 96, 97 and 98% respectively.