Regulation of aflR and its product, AfiR, associated with aflatoxin biosynthesis.
LIU, B.H. and CHU, F.S.
Applied and Environmental Microbiology 64: 3718 – 3723.
1998
บทคัดย่อ
The role of the regulatory gene aflR and its product, AfiR , in the biosynthesis of aflatoxin in Aspergillus was examined. Western blot and ELISA analyses revealed that AFB1 accumulation was directly related to AfiR expression and was regulated by various environmental and nutritional conditions, including temperature, air supply, carbon source, nitrogen source and zinc availability, Expression of an aflatoxin biosynthetic pathway structural gene, omtA, was regulated by the presence of AfiR. Induction patterns for aflR mRNA nd AfiR were correlated with that for omtA mRNA in an aflatoxin – producing strain of Aspergillus parasiticus. Analysis of the non aflatoxin producing strains of A.flavus, A.sojae and A.oryzae showed that both aflR mRNA and AfiR production were present, however, omtA mRNA production was not detected. AflR in the A. oryzae strain was regulated by carbon source and temperature in a manner similar to that seen with A .parasiticus.