บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin.

MEYERS, D.M., OBRIAN, G.,DU, W.L., BHATNAGAR, D. and PAYNE, G.A.

Applied and Environmental Microbiology 64: 3713 – 3117.

1998

บทคัดย่อ

Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin.

The genes encoding the aflatoxin biosynthetic pathway enzymes have been localized as a cluster to a 75 kb DNA fragment. Reported here is the characterization of one of these genes, a gene designated aflJ. Disruption of aflJ in Aspergillus flavus results in a failure to produce aflatoxins and a failure to convert exogenously added path – way intermediates norsolorinic acid, sterigmatocystin, and O – methylsterigmatocystin to aflatoxin. The disruptied strain does, however, accumulate pksA, nor1, ver1 and omtA transcripts under conditions conducive to aflatoxin biosynthesis. AflJ does not appear to have a regulatory function similar to that of aflR. Sequence analysis of aflJ and its putative peptide, AflJ, did not reveal any enzymatic domains or significant similarities to proteins of known function.