บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Purification and quantification of deatruxins A and B from Metahizium anisopliae.

CHEN,J.W.,LIU,B.L.and TZENG,Y.M.

Jounal of the Chromatography A 830: 115-125

1999

บทคัดย่อ

Purification and quantification of deatruxins A and B from Metahizium anisopliae.

Purification of the insecticidal cyclodepsipeptides, destruxins A (DA) and B (DB), from Metarhizium anisopliae fermentation broth was performed.  The destruxins were isolated using ion exchange chromatography, silica gel chromatography and semi-preparative HPLC chromatography.  Over 90% purity was achieved for both DA and DB.  The purified destruxins were further identified by [1H] NMR and fast atom bombardment MS.  HPLC quantification methods for both destruxins in fermentation broth have been established.