ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins.

ENGLER, K.H., COKER, R. and EVANS, I.H.

Journal of Microbiological Methods 35 : 207-218.

1999

บทคัดย่อ

A novel colorimetric microbial bioassay showing particular sensitivity to trichothecenes is described.  The assay uses inhibition of expression of beta-galactosidase activity within the yeast Kluyveromyces marxianus as a sensitive toxicity indicator, cultures remaining yellow, rather than turning deep green-blue, in the presence of X-gal, a chromogenic substrate.  The assay is conducted in standard microtitre plates and can be scored either automatically by a platereader, or by eye.  Polymyxin B nonapeptide was the most effective toxicity-enhancing membrane modulating agents (MMA) tested, enabling verrucarin A to be detected at a concentration of about 1 mg/L.  Using polymyxin B sulfate as an alternative MMA, and T2 toxin, reproductibility and sensitivity were better for the beta-galactosidase X-gal endpoint than for an alternative chromogenic toxicity indicator, the respiratory substrate MTT.