บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Characterization of the promoter for the gene encoding the aflatoxin biosynthetic pathway regulatory protein AFLR

EHRLICH, K.C., CARY, J.W. and MONTALBANO, B.G.

Biochimica et Biophysica Acta – Gene Structure and Expression 1444 : 412-417.

1999

บทคัดย่อ

Characterization of the promoter for the gene encoding the aflatoxin biosynthetic pathway regulatory protein AFLR.

Most genes in the aflatoxin biosynthetic pathway in Aspergillus parasiticus are regulated by the binuclear zinc cluster DNA-binding protein AFLR.  The aflR promoter was analysed in beta-glucuronidase reporter assays to elucidate some of the elements involved in the gene’s transcription control.  Truncation at 118 bp upstream of the translational start site increased promoter activity 5 fold, while truncation at –100 reduced activity about 20 fold.  These findings indicate the presence of an important positive regulatory element between-100 and –118 and a negative regulatory region further upstream.