บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Production and characterization of monoclonal antibodies against norsolorinic acid reductase involved in aflatoxin biosynthesis.

LEE, R.C. and CHU, F.S.

Food and Agricultural Immunology 11 : 29-42.

1999

บทคัดย่อ

Production and characterization of monoclonal antibodies against norsolorinic acid reductase involved in aflatoxin biosynthesis.

Norsolorinic acid reductase (NSR) is responsible for the conversion of norsolorinic acid to averantin in the early stage of aflatoxin biosynthesis.  Using a partially purified NSR preparation as the immunogen, monoclonal antibodies (mAbs) against NSR were produced.  An ELISA, using partially purified NSR as coating antigen and a second antibody-peroxidase conjugate as indicator, was established for measurement of the antibody titre and enzyme level.  HPLC-postcolumn ELISA, immunoblot analysis and enzyme inhibition study revealed that a mAb elicited in cell line 10D2 specifically bound with the 43 kDa NSR.  The mAb produced by 10D2 cell line was capable of partially neutralising the NSR activity and has also been used for detection of expression of the gene encoding the enzyme..