Determination of fumonisins B1and B2 and their major hydrolysis products in corn, feed, and meat, using HPLC.
THAKUR, R.A. and SMITH, J.S.
Journal of Agricultural and Food Chemistry 44 : 1047-1052.
1996
บทคัดย่อ
A HPLC coupled to a fluorescence detector was used to analyse 0-phthaldialdehyde derivatised FB1, B2, hydrolysed FB1, hydrolyzed B2 and partially hydrolysed FB1 from corn, feed and spiked meat samples. Chromatography was achieved on a C18 reversed phase column. The mobile phases were acetonitrile-water-acetic acid mixtures set at A (40:59:1) and B (60:39:1), pumped in a gradient of 100% A to 100% B (9 min), and followed by a step-up to 100% acetonitrile © at the end of 17 min. The HPLC method has a detection limit of 20mg/L.