Assessment of immunochemical methods for the analysis of trichothecene mycotoxins in naturally occurring moldy corn.
PARK, J.J. and CHU, F.S.
Journal of AOAC International 79 : 465-471.
1996
บทคัดย่อ
The efficacies of various immunochemical methods for simultaneous analysis of different trichothecenes in naturally contaminated corn samples were evaluated. Antibodies against either T-2 tetraol tetraacetate (T-2-4ol-4Ac) or DON triacetate were used in the ELISA and radioimmunoassay (RIA) for total type P and total DON related trichothecenes, respectively. Specific antibodies against T-2 toxin were used in an RIA for analysis of T-2 toxin alone. Analytical recoveries of T-2 toxin analysed as T-2-4ol-4Ac by ELISA and T-2 toxin by RIA at 10-200mg/kg were 83.5-123% and 81.8-110.6%, respectively. T-2 toxin accounted for about 30% of total type A trichothecenes. A good correlation was found for T-2 toxin levels obtained by LC-ELISA and RIA methods. In addition to T-2 toxin, HT-2 toxin, diacetoxyscirpenol and T-2 tetrol were found in samples containing high levels of type A trichothecenes by LC-ELISA. For analysis of DON alone, samples were treated with a SepPak C18 cartridge to remove other acetylated DONs before RIA. Recovery of DON after the C18 cartridge treatment was 90%. Overall analytical recoveries of DON as DON triacetate (sample with no C18 treatment) and DON alone (with C18 treatment) at 10-500mg/kg were 81.3-94% and 72.7-104%, respectively. RIA revealed that DON accounts for about 50% of total DON related trichothecenes.