บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Use of a heterologous bridge coating antigen for the immunoassay of 3-acetyldeoxynivalenol in barley.

WANG, N., KIEREKJASZCZUK, D., MARQUARDT, R.R., FROHLICH, A.A. and ABRAMSON, D.

Journal of Food Protection 59 : 525-533.

1996

บทคัดย่อ

Use of a heterologous bridge coating antigen for the immunoassay of 3-acetyldeoxynivalenol in barley.

A highly sensitive ELISA for the quantitation of 3-acetylDON in barley is described.  Polyclonal antibodies were produced in rabbits immunised with a conjugate of 3-acetylDON and human serum albumin linked by an ester linkage (hemisuccinate bridge).  However, only a negligible degree of inhibition was obtained with 3-acetylDON in a competitive ELISA when the coating conjugate contained the same ester linkage group (hemiglutarate bridge) as the immunogen.  The use of a conjugate containing a heterologous ether linkage (0-methylcarboxyl bridge) compared to that of the immunogen yielded an inhibition curve for 3-acetylDON that was highly sensitive with essentially no interference from the bridging group.  This conjugate was synthesised using iodoacetate and 1,1’ –carbonylidiimidazole chemistries.  The ELISA developed on the basis of this new conjugate was able to detect 3-acetylDON at 16mg/kg in spiked barley without any cleanup treatments.