บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Determination of deoxynivalenol in wheat, barley, and malt by column cleanup and gas chromatography with electron capture detection.

TACKE, B.K. and CASPER, H.H.

Journal of AOAC International 79 : 472-475.

1996

บทคัดย่อ

Determination of deoxynivalenol in wheat, barley, and malt by column cleanup and gas chromatography with electron capture detection.

A rapid and reliable method to determine DON in wheat, barley and malt is described.  Samples are extracted with acetonitrile-water (84+16).  Extracts are eluted through a C18-alumina (1+3) column, evaporated to dryness, and derivatised with trimethylsilylimidazole-trimethylchlorosilane (100+1).  DON is identified and quantitated by capillary gas chromatography with electron capture defection.  This method can quantitate DON levels from 0.2-40mg/kg.  Recoveries of DON from spiked samples were 94-100% for wheat, 100-105% for barley and 100-105% for malt.  Results compared well with results for the same sample analysed by ELISA and LC.