Effect of high CO2 pretreatment on quality, fungal decay and molecular regulation of stilbene phytoalexin biosynthesis in stored table grapes
M Teresa Sanchez-Ballesta, Jorge Bernardo Jiménez, Irene Romero, José M Orea, Roberto Maldonado, Ángel González Ureña, M Isabel Escribano and Carmen Merodio
Postharvest Biology and Technology, Volume 42, Issue 3 , December 2006, Pages 209-216
2006
บทคัดย่อ
Table grapes (Vitis vinifera) cv. Cardinal stored at low temperature were analysed to determine the effect of pretreatment with 20 kPa O2 + 20 kPa CO2 + 60 kPa N2 for 3 days on quality and control of decay. The pattern of stilbene synthase (STS) gene expression and trans-resveratrol levels were also analyzed in grapes during low temperature storage at 0 °C and further shelf-life at 20 °C for 2 days. Our results showed that high CO2 pretreatment was effective for improving appearance of the bunches and maintaining the quality of the berries. In CO2-treated bunches the browning and withering index, the decline in relative water content and the weight loss were also lower than in non-treated ones. The levels of STS mRNA and the accumulation of trans-resveratrol in CO2-treated grapes were much lower than in the non-treated grapes during low temperature storage. Moreover, the pattern of STS gene expression and trans-resveratrol content in CO2-treated grapes was consistent with the reduction of natural total decay produced by this pretreatment. This effective non-stressing treatment avoids the induction of trans-resveratrol during low temperature storage until its synthesis is enhanced during shelf-life at 20 °C.