Fruit ripening characteristics in a transgenic ‘Galia’ male parental muskmelon (Cucumis melo L. var. reticulatus Ser.) line
Hector G. Nuñez-Palenius, Donald J. Huber, Harry J. Klee and Daniel J. Cantliffe
Postharvest Biology and Technology, Volume 44, Issue 2, May 2007, Pages 95-100
2007
บทคัดย่อ
‘Galia’ is a high-quality muskmelon cultivar that is grown in greenhouses or tunnels to maximize fruit quality and yield. ‘Galia’ has a short shelf life of 2–3 weeks due to rapid fruit softening. In vitro regeneration and transformation of ‘Galia’ melon parental lines with antisense technology, targeting enzymes involved in the ethylene biosynthesis pathway, is a feasible strategy that can be used to increase its fruit shelf-life. In this study, the male parental line of ‘Galia’ muskmelon was transformed with two different constructs: one plasmid was bearing the uidA (GUS) reporter gene and another the ACC oxidase gene (CMACO-1) in antisense orientation. Transgenic ACC oxidase antisense (TGM-AS), azygous (PCR negative), transgenic GUS (TGM-GUS) and wild type (WT) fruit, from plants grown in the greenhouse, were harvested at zero-, half-, and full-slip developmental stages. Fruit firmness of full-slip TGM-AS was almost twice that of wild type, azygous and TGM-GUS. Ethylene production and ACC oxidase in half-slip wild type, azygous and TGM-GUS fruit were greater than those from TGM-AS fruit. TGM-AS ‘Galia’ male parental melon fruit exhibited delayed softening compared to wild type fruit.