บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Prickly pear polygalacturonase gene: cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding

Flor de Fátima Rosas-Cárdenas, María Leonor Valderrama-Cháirez, Andrés Cruz-Hernández and Octavio Paredes-López

Postharvest Biology and Technology, Volume 44, Issue 3, June 2007, Pages 254-259

2007

บทคัดย่อ

Prickly pear polygalacturonase gene: cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding

Polygalacturonase (PG; EC 3.2.1.6.9) has been the most widely studied cell wall hydrolase in fruit ripening. Degenerate oligonucleotides corresponding to conserved regions from reported sequences were used as primers for RT-PCR to amplify mRNA extracted from middle ripe fruit. Cloning and characterization of a cDNA OsPG showed a 282 bp product with a predicted sequence of 94 amino acids. The peptide exhibited a high identity with previously reported fruit PGs. Northern blot analysis of the messenger showed a 1.7 kb transcript induced during prickly pear ripening. It was found by Southern blot analysis that there is o­ne copy of this gene. The OsPG mRNA expression is sensitive to ethylene, cold storage and wounding.