Detection and identification of seed-borne fungi of pepper (Capsicum annuum).
M.H. Ali, A.M. bdelmonem and M.R. Rasmy.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino, Italy,. 507 pages.
2008
บทคัดย่อ
Detection and identification of seed-borne fungi of pepper (Capsicum annuum).
Sixteen pepper (Capsicum annuum L.) seed samples were collected from Alexandria markets and examined for the presence of seedborne fungi using the standard blotter and agar plate methods (ISTA, 1981). We found that Alternaria alternata, Aspergillus spp., Cladosporium herbarum, Colletotrichum capsici, Curvularia lunata, Fusarium oxysporum, F. semitectum, F. solani, Penicillium spp., Rhizopus sp., Rhizoctonia solani and Stemphylium sp. were the predominant fungi associated with pepper seeds. The standard blotter method was better than the agar plate method as it detected 11 fungi compared to 8 fungi. Pathogenicity tests revealed that some of the pepper seedborne fungi could produce damping-off and wilt on the pepper cultivars tested. Seed infection levels of pepper with F. oxysporum had a significant effect on wilt incidence. Culture filtrate either of F. oxysporum or F. solani significantly reduced pepper seed germination as compared to F. semitectum. By means of specific primers, the F. oxysporum was identified as f. sp. capsici.