Detection of Penicillium expansum associated with blue mould on apples in Ontario using PCR-RFLP.
D. Errampalli, V. Popovic and K. Ellens.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Detection of Penicillium expansum associated with blue mould on apples in Ontario using PCR-RFLP.
Blue mould (caused byPenicilliumspp.) is one of the most important postharvest diseases of apples in southernOntario,Canada.AlthoughPenicillium expansumis the most common and aggressive species, several others such asP. brevicompactum,P. crustosum,P. aurantiogriseum,P. polonicum,P. viridicatum, andP. solitumare also known to cause blue mould symptoms.The objective of this study was to identify species ofPenicilliumpresent among fifty six isolates in three apple packinghouses in southernOntario. Genotypic species identification was performed by the restriction fragment length polymorphism (RFLP) analysis of a polymerase chain reaction (PCR) amplified product from the internal transcribed spacer (ITS4 and ITS5) region of rDNA. Digestion of the ~600 bp DNA fragment, amplified from the ITS region, using restriction enzymesHinfI andTaqI revealed distinctive banding patterns:HinfI produced three bands (300, 200, and 100 bp) forP. expansum, and two bands (325 and 275 bp) forP. solitum, whileTaqI produced three bands (275, 160, and 150 bp) forP. expansum, and four bands (190, 180, 170, and 60 bp) forP. solitum. In one exception,TaqI produced two bands (325 and 275 bp) forP. brevicompactum. The PCR-RFLP technique was successful in differentiating between all but two of the above mentionedPenicilliumspp. Based on PCR-RFLP analysis, 53 isolates were identified asP.expansum, and 3 asP. solitum. Pathogenicity tests showed thatP. expansumwas more aggressive thanP. solitumon apple ‘McIntosh’.