Molecular and immunological detection of fusarium infection and fumonisin contamination in major food crops in southern India
S. Chandra Nayaka, A.C.Uday Shankar, S.R Niranjana and H.S.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Molecular and immunological detection of fusarium infection and fumonisin contamination in major food crops in southern India
Fumonisins, are group of mycotoxins mainly produced byFusarium verticillioidesandF. semitectum, have adverse health effects on humans and livestock that ingest fumonisin-contaminated food products and feeds. Maize, sorghum and rice are excellent substrates for the growth ofFusariumsp., and fumonisin (FB) production. In order to understand the magnitude ofF. verticillioidesandF. semitectuminfection and FB contamination, surveys were conducted in different agro-ecological zones of southernIndia, and 112 samples of maize, rice and sorghum were collected from different sources. These samples were analyzed for FB1 and FB2 contamination by HPLC and Competitive Direct ELISA. Analysis indicated that post-harvest was the favorable stage for infection byF. verticillioidesandF. semitectumand FB production. More than 60 % of the maize kernels, 30% sorghum seed samples and 23% of rice seed samples tested were contaminated with FB, ranging from 3to 927μg/kg. A PCR for specific detection of toxigenic and non-toxigenicF. verticillioideswas standardized. Specific primers VERT1, VERT2 and for toxigenic strains, VERTF1 and VERTF2 were designed based on IGS sequence.The primers specific to the toxigenicF. verticillioideswere highly effective and amplified the expected 400 bp product.This study demonstrated the potential utility of PCR using specific primers for detection ofF. verticillioidesin major food crops.