F. Ciccarese, T. Ziadi, A. Ambrico, A. Ciccarese, M. Sciacovelli and M. Gallo.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Use of ozone in seed decontamination
Surface contamination of seed by pathogenic and/or saprophytes fungi is considered a serious problem because disease may be transmitted or because dangerous mycotoxins may be carried.The mycotoxines (aflatoxin B1, B2, G1, G2, M1, ocratoxin A, fumonisin, zearalenone, etc.) are produced byAspergillusspp.,Penicilliumspp., Fusariumspp., etc. Ozone is a biocide used widely for microbial decontamination. It is classified as a safe substance “GRAS” (Generally recognized as safe) by the U.S. Food and Drug Administration. We report the results of a trial of ozone treatments in seeds decontamination. A local variety of pea seed collected in Southern Italy from a grower producing the seed himself for sowing, a 1-year old sample of stored wheat grain, cv. Cappelli, and barley grain, cv. Jiaidor, were tested.Ozone at a concentration of 4 ppm was applied as a mixture of ozone + air for 1, 1.5 and 3 minutes. Untreated seeds were used as control. Treated and untreated seeds were analyzed and the fungi developed were identified and counted.Fusariumspp.,Alternariaspp.Penicilliumspp. andAspergillusspp. were observed in most cases. Exposure to ozone for 1 min was partially effective, whereas treatment for 3 min gave good seed decontamination without any influence on germination.