Food industry by-products as raw material for production of the biocontrol agent Pantoea agglomerans PBC-1
T. Manso, C. Nunes, D. Neto, J. Pardão, S. Raposo and E. Lima-Costa.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Food industry by-products as raw material for production of the biocontrol agent Pantoea agglomerans PBC-1
Pantoea agglomeransPBC-1 was originally isolated from thesurface of oranges and can control postharvest pathogens in pomeand citrus fruits. The use of a biocontrol agent depends on developinga culture medium which can produce large amounts at lowcost. In this study, food industry by-products were investigated toassess their potential as a raw material for production of the biocontrolagentPantoea agglomeransPBC-1. Carob extract, sugarbeet molasses, orange by-products, and commercial sugar wereused as carbon source. Viable populations were determined at theend of 24 h incubation at 30ºC under orbital agitation at 150 revmin-1. No differences were observed between the various mediastudied when compared with the standard media; the viable populationsranged between 1x109an3x109cfu.ml-1. The biomassproduced in the different media was then evaluated against bluemould of apple fruit. Fruits were wounded artificially, treated withaP. agglomeranssuspension at 1x108cfu.ml-1and infected withPenicillium expansumat 1x104spores.ml-1.The greatest reductionin lesion diameter (99%) and incidence (95%) were obtained bytreating withP. agglomeransproduced with carob extract. Thebiomass productivity and efficacy against the pathogen obtainedwith the different by-products show that these industrial by-productscan be used efficiently to produce biocontrol agents.