Inactivation of Penicillium expansum polygalacturonase using antibody technology
J.L. McEvoy, W.J. Janisiewicz and W.S. Conway.
Journal of Plant Pathology Volume 90 (2, Supplement) August 2008, Book of Abstract, 9th International Congress of Plant Pathology, August 24-29, 2008 Torino,Italy,. 507 pages.
2008
บทคัดย่อ
Inactivation of Penicillium expansum polygalacturonase using antibody technology
The blue mould pathogen,Penicillium expansum, producespolygalacturonase (PG) during decay of apples in storage. PG, believed to be a major virulence factor, is produced in a medium-dependent fashion. Four or more isozymes are produced in culture depending on the medium but only one major and one minor isozyme are produced in apple tissue. The invivo-produced isozymes were purified using ammonium sulfate precipitation, gel filtration and ion exchange techniques.Fractions from the two peaks of activity separated by gel filtration were used in generating anti-PG polyclonal antibodies in mice. Antibodies raised against the major PG peak were found to inhibit enzyme activity by up to 60% inin vitroassays. Current work, which includes enzyme inactivation on fruit (and its effect on virulence) and the generation and use of recombinant antibodies to determine the role of individual isozymes in disease development, will be discussed.