Abstracts of 27th International Horticultural Congress & Exhibition (IHC 2006), August 13-19, 2006, COEX (Convention & Exhibition), Seoul, Korea. 494 pages.
2006
บทคัดย่อ
Characterization of Mg-dechelation activity with chlorophyll degradation in stored broccoli florets
Characterization of Mg-dechelation activity with chlorophyll degradation of stored broccoli (Brassica oleracea L.) florets was performed to clarify the mechanism of chlorophyll degradation.The Mg-dechelation activity of an extract from yellow broccoli, which was stored for 4 days at 15°C, was compared to that of fresh broccoli using chlorophyll in a as substrate.The pH optimum and stability of both extracts were 8.0 and 8.5, respectively.The temperature stability was 40°C in both extracts.Chelating compound, radical scavengers and reducing agents had different inhibitory affects.Ascorbate, and especially quercetin, had more effective inhibitory activity on both extracts.Two different molecular weight substances, one a low molecular weight (5,000-10,000) type and the other a high molecular weight (more than 10,000) type, were present in the extracts.The Mg-dechelation activity of both molecular weight types in yellow broccoli extract was higher than in fresh broccoli extract, suggesting that Mg-dechelation activity could be involved in chlorophyll degradation of stored broccoli florets.