Antagonistic action of siderophore produced from Burkholderia sp. CAS-5 upon the postharvest pathogen Penicillium expansum
Kyoung-Ja Kim and Yong-Joom Yang
Abstracts of 27th International Horticultural Congress & Exhibition (IHC 2006), August 13-19, 2006, COEX (Convention & Exhibition), Seoul, Korea. 494 pages.
2006
บทคัดย่อ
A soil microorganism producing sideropoure under low iron stress (up to 5 μM of iron) was identified as Burkholderia sp. by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron microscopy.Hydroxamate nature of siderophore was detected by Csaky test.Hydroxamate type siderophore produced by Burkholderia sp.CAS-5 was partially purified by extraction the supernatant with methanol and Sephadex LH-20 column chromatography with methanol as eluent.The purified siderophore improved the biological control of blue rot caused by Penicillium expansum.The production of the siderophore was closely associated with the iron concentration in the medium.Thus, very low additions of the iron reduced the siderophore production considerably.The antagonistic effect of Burkholderia sp. CAS-5 and siderophore against P. expansum was studied by using in vitro assay.In the in vitro assay, siderophore reduced the growth of P. expansum, whereas the chelate (siderophore plus iron) did not.Siderophore antagonism was then related to competition for iron.