Determination of Volatile Constituents of Longan Arillus, Epicarpium and Semen by Gas Chromatography-mass Spectrometry Coupled with Solid-phase Microextraction
Chen Faxing, Wu Shaohua, Zheng Shenxi, Sheng Suanling, Li Yongyu, Wen Fenyen and Li Xingle
Program and Abstracts, 3rd International Symposium on Longan, Lychee and Other Fruit Trees in Sapindaceae Family, August 25-29, 2008, Fuzhou, China. 132 pages.
2008
บทคัดย่อ
Determination of Volatile Constituents of Longan Arillus, Epicarpium and Semen by Gas Chromatography-mass Spectrometry Coupled with Solid-phase Microextraction
Gas chromatography-mass spectrometry combined with solid-phase microextraction (SPME-GC-MS) was developed for the determination of volatile compounds of arillus, epicarpium and semen. The volatile constituents of longan were extracted by SPME with a 100µm polydimethylsiloxane fiber. The fibers were desorbed in a GC injection liner at 250°C for 5 min. With headspace SPME-GC-MS analysis, 74 peaks in longan arillus were separated and identified by mass spec-trometry. The principal components were Bicyclo[4.4.0]dec-2-ene-4-ol,2-methyl-9-(prop-1-en-3-ol-2-yl)-(81.69%);1H-Cycloprop[e]azulene,1a,2,3,4,4a,5,6,7b-octahydro-1,1,4,7-tetramethyl-,[1aR-(1aα,4α,4aß,7bα)]-(3.16%);Tricyclo[3.3.0.0(2,8)]octan-3-one,7-hydroxy-4-methly-4-(propan-2-on-1-yl)-(1.91%);2,6,10-Dodecatrienoic acid, 3,7,11-trimethyl-, methyl ester (1.26%) and Diethyl Phthalate (1.05%). A total of 54 compounds were identified in longan semen. The main components were 1 H-Cycloprop[e]azulene,1a, 2, 3, 4, 5, 6, 7b-octahydro-1,1,4,7-tetramethyl-,[1aR-(1aα,4α,4aß,7bα)]-(30.38%); aryophyllene(18.04%);Azulene,1,2,3,4,5,6,7,8-octahydro-1,4-dimethy1-7-(1-methylethenyl)-,[1s-(1α,4α,7α)]-(5.21%);3-Nonyne(5.15%), Benzene,cyclohexyloxy)(4.92%);Naphthalene,1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethy-7-(1-methylethenyl)-,[1R-(1α,7ß,8aα)]- (3.79% and 3-Carene (3.13%). Varieties of 90 compounds were identified in the longan epicarpium extracted by SPME. The main components were Gitoxigenin (47.59%); ß-D-Galactopyranoside,methy12,3-bis-0-(trimethylsilyl)-,cyclicbutylboronate(22.92%);Columbin(7.76%);Benzene,1,1'-[3-(3-cyclopentlypropyl)-1,5-pentanediyl]bis-(6.21%) and Resibufogenin(3.39%). This was the first report describing the quantitative and qualitative both differences of volatile composition in longan fruit.