Biological control of postharvest blue mold of oranges by Cryptococcus laurentii (Kufferath) Skinner
Hong-yin ZHANG, Xiao-dong ZHENG and Yu-fang XI
BioControl 50 (2): 331-342. 2005.
2005
บทคัดย่อ
Biological control of postharvest blue mold of oranges by Cryptococcus laurentii (Kufferath) SkinnerCryptococcus laurentii (Kufferath) Skinner was evaluated for its activity in reducing postharvest blue mold decay of oranges caused by Penicillium italicum in vitro and in vivo. The results showed that washed cell suspensions of yeast provided control of blue mold decay better than yeast in culture broth. Autoclaved cell culture and cell-free culture filtrate failed to provide protection against the pathogen. The concentrations of antagonist had significant effects on biocontrol effectiveness. When the washed yeast cell suspension reached the concentration of 1 × 109 CFU/ml, challenged with pathogen spore suspension at 1 × 104 spores/ml, the blue mold decay was completely inhibited during 5 days of incubation at 20 °C. No complete control was obtained when oranges were stored at 4 °C for 30 days, but the decay was distinctly prevented. Efficacy of C. laurentii was maintained when applied simultaneously or prior to inoculation with P. italicum. Efficacy was reduced when C.laurentii was applied after inoculation. In drop-inoculated wounds of oranges, the populations of C.laurentii increased by approximately 50-fold during the first 24 h at 20 °C. The maximum yeast populations, approximately 250-fold over the initial populations, were reached 15 days after inoculation at 4 °C.