Introduction of ethylene insensitivity in Campanula carpatica flowers by genetic manipulation with Etr1-1
Sridevy Sriskandarajah, Heiko Mibus and Margrethe Serek
Abstracts Book, 6th International Postharvest symposium, 8-12 April 2009, Antalya, Turkey. 256 pages.
2009
บทคัดย่อ
The construct containing the etrl-l gene from Arabidopsis thaliana under control of the flower specific jbpl-promoter from Petunia was used to obtain Campanula carpatica Jacq. potted plants with ethylene insensitive flowers. The flowers on the wild type Campanula plants wilted within three days when exposed to 2 µl l-1 ethylene. The tested transgenic etrl-1 lineshad various levels of tolerance against ethylene and the best transgenic line continued to flower for up to 27 days in the presence of exogenous ethylene. The expression of etrl-l in flowers and buds was cOnlumed by PCR and RT-PCR. The transgenic plants were fertile without any reduction in rooting ability of cuttings, and they did not differ morphologically from the wild type. Tl progeny, a cross between a wild plant and a transgenic plant possessing ethylene insensitive flowers, showed 1:1 segregation in terms of ethylene insensitivity and the presence of the trans gene.