ความหลากหลายทางพันธุกรรมของยีน lycopene -cylase และ β-carotene hydroxylase ในมะละกอสายพันธุ์การค้า
พิมพิไล แสงมณี ธนพล ไชยแสน สิริกุล วะสี และ ปาริชาติ เบิร์นส
วารสารวิทยาศาสตร์เกษตร 44 (3พิเศษ): 113-116. 2556.
2556
บทคัดย่อ
Papaya (Carica papaya L.) is an important cash crop for local consumption and export. Nowadays, consumers and food manufacturers require several horticultural features particularly flesh colour; red and yellow. However, the lack of standard in commercialized papaya cultivar results in variation in colour. Papaya colour is found associated with carotenoid biosynthetic pathway. In this study, fifteen papaya cultivar including cultivar in breeding program, commercial and local cultivar were used and their flesh colour were determined. Fifty-nine gene specific primers were designed for each of carotenoid synthetic gene and used to amplifiy DNA fragment with approximate size of 400-600 base pairs and DNA fragment migration pattern was determined by denaturing acrylamide gel electrophoresis. The results showed that fifty-six gene specific primers were able to amplified carotenoid synthetic genes. DNA polymorphism via migration pattern was found in several carotenoid synthetic genes including lycopene e-cylaseand β-carotene hydroxylase. The measurement and analysis based on L*, a* and b* indicated that papaya flesh colour can be divided into 3 groups; red, orange and yellow. Information will lead to the development of the molecular markers associated will the color of flesh papaya in the future.