Role of hydroxyl radical in modification of cell wall polysaccharides and aril breakdown during senescence of harvested longan fruit
Xuewu Duan, Haiyan Zhang, Dandan Zhang, Jiangfeng Sheng, Hetong Lin and Yueming Jiang
Food Chemistry, Volume 128, Issue 1, 1 September 2011, Pages 203-207
2011
บทคัดย่อ
The effects of reactive oxygen species (ROS), especially hydroxylradical (•OH), on cellular wall disassembly in vitro and in vivo, and aril breakdown in longanfruit, were investigated. •OH caused the disassembly of cellwall material (CWM) in vitro, demonstrated by the increases of total sugars and uronic acid released, and the downshift in molecular mass of CDTA soluble pectin (CSP) and Na2CO3 soluble pectin (CO3-SP). In addition, •OH resulted in decreased CO3-SP content and increased CSP content in CWM suspension, suggesting the conversion of CO3-SP to CSP. Application of exogenous •OH accelerated arilbreakdown in longanfruit while the process was delayed by l-cysteine·HCl, a ROS scavenger. Furthermore, lower CWM content and decreased molecular mass of pectins were observed in •OH-treated fruit. These results indicated that •OH contributed to the degradation of cell wall polysaccharides, resulting in aril breakdown in longanfruit.