Solid-phase extraction and HPLC determination of Ochratoxin A in cereals products on Chilean market
Mario Vega, Katherine Muñoz, Carolina Sepúlveda, Mario Aranda, Victor Campos, Ricardo Villegas and Orialis Villarroel
Food Control, Volume 20, Issue 7, July 2009, Pages 631-634
2009
บทคัดย่อ
Ochratoxin A(OTA) is a mycotoxin produced by different species of Aspergillus and Penicillium fungi. The presence of this mycotoxin in cereals-based products has relation with manufacturing practices, especially with storage conditions. An extraction procedure for OTA from wheat-based products was implemented in this study. The method uses an alkaline extraction with NaHCO3, purification with Sep-Pak® RP-18 cartridges; and quantitative analysis by high performance liquid chromatography with fluorescence detection. The presence of OTA was confirmed by the formation of Ochratoxin A methyl ester. The method shows good validation parameters with a rate of recovery rate over 95%, limits of detection and quantification of 0.6 and 2.1 μg kg−1, respectively. Once the method was validated; 31 samples including, flour, corn starches and rice were analyzed. About 70% of flour samples, 50% of rice and 63% of corn starch samples resulted positives for OTA.