Integrated control of crown rot of banana with Candida oleophila strain O, calciumchloride and modified atmosphere packaging
Héloïse Bastiaanse, Luc de Lapeyre de Bellaire, Ludivine Lassois, Coralie Misson and M. Haïssam Jijakli
Biological Control,Volume 53, Issue 1, April 2010, Pages 100-107
2010
บทคัดย่อ
An integrated approach for biological control of crown rot of banana was studied. The efficacy of three control measures, applied alone or in various combinations, was evaluated under conditions highly conducive to the development of crown rot (artificial inoculation of Colletotrichummusae at 104conidia/ml). The studied measures were: application of an antagonistic yeast (Candidaoleophila strain O at 1.107cfu/ml), treatment with 2% (w/v) calciumchloride, and modified atmosphere packaging of fruit (MAP) in non-perforated polyethylene bags. C. oleophila was able to grow under MAP, maintaining a large population (7.106 to 7.107cfu/g crown) throughout the 13 days of storage. Both treatment with the antagonistic yeast and storage under MAP, applied separately, reduced crown rot significantly (by 22% and 20%, respectively, as compared to untreated controls). The effect of the yeast was the same whether it was produced in Petri dishes or in a fermentor. Calciumchloride treatment alone had no effect on C. musae. The antagonistic yeast showed a 16% higher biocontrol activity (from 26% to 42%) when applied together with 2% (w/v) calciumchloride, and the presence of this adjuvant made it possible to achieve the same protective effect with a lower yeast concentration. The highest efficacy (53%) was achieved by the combination of the three alternatives means of control and a synergistic relation has been detected between the yeast, calciumchloride and MAP. Considering the severe conditions of screening, the consistency of the results obtained in this study indicates that the integrated strategy has great potential for control of crown rot of banana under commercial conditions.