Antifungal effects of chitosan with different molecular weights on in vitro development of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill
A.N. Hernández-Lauzardo, S. Bautista-Baños, M.G. Velázquez-del Valle, M.G. Méndez-Montealvo, M.M. Sánchez-Rivera and L.A. Bello-Pérez
Biological Control,Volume 73, Issue 4, 5 September 2008, Pages 541-547
2008
บทคัดย่อ
Determination of the molecular weight of three types of chitosan was carried out by HPSEC-RI. The effect of low, medium and high molecular weight chitosan was evaluated on development of three isolates of Rhizopusstolonifer. Image analysis and electronic microscopy observations were done in spores of this fungus. Germination of R. stolonifer in potato dextrose broth with chitosan was also evaluated. Results pointed out that the low molecular weight chitosan was more effective for inhibition of mycelial growth while the high molecular weight chitosan affected spore shape, sporulation and germination. Studies of scanning and transmission electron microscopy revealed numerous and deeper ridge ornamentations of the chitosan-treated spore.