In- vitro propagation of Alstroemeria plant by tTCL and lTCL techniques
M. Arshad, E. Chamani and Y. Pourbeyrami
Book of Abstracts.International Conference on Quality Management in Supply Chains of Ornamentals. 21-24 February, 2012. Golden Tulip Sovereign Hotel, Bangkok, Thailand.
2012
บทคัดย่อ
Two different experiments were done to study the regeneration of rhizome and shoot in Alstroemeria aurantifolia 'Konyambe' flower by tTCL and lTCL techniques under in vitro condition in biotechnologylaboratory of Mohaghegh Ardabili University in Iran.Experiments were arranged in completely randomized designs with five replications.The results of experiments showed that this cultivar of Alstroemeria was not competent to produce somatic embryogenesis by tTCL and lTCL. The results also showed rhizome of Alstroemeria gave the most and highest in-vitro regeneration compared to other organs. No regeneration was found in stem explantswhich micro-propagated by tTCL and lTCL techniques. Among all evaluated traits, significant difference (P<0.05) was found only in rhizome number whichL2D1treatment was produced the highest rhizome number (mean=3 numbers) compared to other treatments. The results of NAA and BA effects on in-vitro regeneration of Alstroemeria flowers revealed that 1ppm NAA in combination with 1ppm BA produced the highest and significant rhizome number (mean= 6.16 numbers) compared to other treatments. However, no significant were found in other traits. Comparision of means revealeted that the highest root number (2.09) was related to 1 ppm NAA in combination with 1 ppm BA. The highest root length (1.38 cm) was obtained from media which contained 0.1 ppm NAA in combination with 2 ppm BA.