Towards prolonging the vase life of cut flowers by slowing their opening: analysis of genes involved in petal cell growth of carnation
S., Satoh, T., Harada, Y. Torii, Y. and S., Morita
Book of Abstracts.International Conference on Quality Management in Supply Chains of Ornamentals. 21-24 February, 2012. Golden Tulip Sovereign Hotel, Bangkok, Thailand.
2012
บทคัดย่อ
Flower opening in carnations (Dianthus caryophyllus L.) is the result of the enlargement of petal cells, which requires sugar metabolism and rearrangement of cell wall constituents. A cDNA encoding sucrose synthase (DcSUS1) was isolated from carnation petals as a candidate gene acting in the sugar metabolism in petal cells. DcSUS1 transcripts accumulated abundantly and nearly constantly in petals of opening flowers. Moreover, four cDNAs encoding xyloglucanendotransglucosylase/hydrolase (XTH) (DcXTH1-DcXTH4) and three cDNAs encoding expansin (DcEXPA1- DcEXPA3) were cloned from petals of carnation flowers. Transcripts of XTHandexpansingenes accumulated differently in tissues of opening carnation flowers, indicating regulated expression of these genes. DcXTH2 and DcXTH3 transcripts were detected in a large amount in petals as compared with other tissues. DcEXPA1 and DcEXPA2 transcripts were accumulated in petals of opening flowers. These findings suggested the involvement of expression of genes for sucrose synthase (DcSUS1), XTH (DcXTH2 and3) and expansin (DcEXPA1 and 2) in petal cell growth during the opening of carnation flowers.