Induction of ethylene insensitivity into Oncidium and Odontoglossum orchid species for improvement of display life
B. Raffeiner, M. Serek and T. Winkelmann
ISHS Acta Horticulturae 906:253-257.2011.
2011
บทคัดย่อ
Two orchid species, Oncidium and Odontoglossum, are sensitive to ethylene. Exposure of cut inflorescences or potted flowering plants to 1 µl L-1 of ethylene accelerated bud drop, wilting of florets, and yellowing of leaves and pedicels. To prevent ethylene effects two strategies have been used in our studies: 1) treatment with the ethylene receptor blocker 1-methylcyclopropene (1-MCP), 2) Agrobacterium mediated transformation with an etr1-1 mutant gene from Arabidopsis thaliana. Selected cultivars of Oncidium and Odontoglossum orchids were pre-treated with 200 nl L-1 1-MCP at 20°C for 6 h and subsequently exposed to 0 or 1 µl L-1 ethylene. 1-MCP clearly improved postharvest characteristics of both investigated species as well in presence as in the absence of ethylene.