Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora
Cladera-Olivera Florencia, Caron Geruza R., Motta Amanda S., André A. Souto and Brandelli Adriano
Canadian Journal of Microbiology, 52(6) p.533-9, 2006.
2006
บทคัดย่อ
Soft rot is a major problem encountered in potatoes during postharvest storage. The soft rot bacterium Erwinia carotovora was inhibited by a novel bacteriocin-like substance (BLS) produced by Bacillus licheniformis P40. The BLS caused a bactericidal effect on E. carotovora cells at 30 μg mL^sup -1^. Transmission electron microscopy showed that BLS-treated cells presented wrinkled bacterial surfaces and shrinkage of the whole cell, indicating plasmolysis. Erwinia carotovora cells treated with BLS were analyzed by FTIR showing differences in the 1390 cm^sup -1^ and 1250-1220 cm^sup -1^ bands, corresponding to assignments of membrane lipids. BLS was effective in preventing E. carotovora spoilage on potato tubers, reducing the symptoms of soft rot at 240 μg mL^sup -1^ and higher concentrations. Soft rot development was completely blocked at 3.7 mg mL^sup -1^. This BLS showed potential to protect potato tubers during storage.