Nano-emulsion of denak (Oliveria decumbens Vent.) essential oil: ultrasonic synthesis and antifungal activity against Penicillium digitatum
Somayeh Rafiee, Asghar Ramezanian, Reza Mostowfizadeh-Ghalamfarsa, Mehrdad Niakousari, Mohammad Jamal Saharkhiz and Elhadi Yahia
Journal of Food Measurement and Characterization 16: 324–331. (2022)
2022
บทคัดย่อ
Denak (Oliveria decumbens Vent.) is an endemic aromatic plant belonging to Apiaceae family. This study evaluated the antifungal activity of denak essential oil (EO) nano-emulsions formulated by ultrasonic emulsification on Penicillium digitatum, the main causal agent of postharvest decay of citrus fruit. EO chemical compositions detected by gas chromatography demonstrated that thymol (28.5%), carvacrol (26.2%), myristicin (17.5%) and elemicin (9.9%) were the dominant components. Denak EO nano-emulsions with droplet diameter of 26.9 nm formulated by ultrasonic emulsification. Inhibition of mycelial growth was observed at EO concentrations higher than 0.5 µL mL−1. The antifungal activity of the EO increased with increasing the concentration of EO. The minimal inhibitory concentration of EO nano-emulsion was found to be 0.5 µL mL−1. These results demonstrate the potential of ultrasonication for preparing denak EO nano-emulsion which could be used as a suitable treatment to control postharvest diseases caused by P. digitatum and possibly other pathogens. This newly formulated denak EO nano-emulsion can be applied as a useful treatment for prevention of citrus losses.