Heat stress alters the transcriptome of Debaryomyces hansenii and reduces its biocontrol activity against postharvest gray mold on kiwifruit
Yuan Dai, Zhenshuo Wang, Jinsong Leng, Qi Wang and Jia Liu
Postharvest Biology and Technology, Volume 178, August 2021, 111541
2021
บทคัดย่อ
Heat stress plays an important role in a postharvest biocontrol system. A basic understanding of heat stress response is crucial to the use of antagonistic yeasts as postharvest biocontrol agents. In the present study, heat stress (42 °C, 20 min) decreased the biocontrol efficacy of the yeast antagonist, Debaryomyces hansenii, against gray mold, caused by Botrytis cinerea, on kiwifruit. RNA-seq was used to conduct a comparative transcriptomic analysis of heat-treated vs. non-heat-treated cultures of D. hansenii. A total of 9775 transcripts comprising 7805 unigenes were obtained from the two treatment groups by de novo assembly. A set of 706 differentially expressed genes (DEGs) were identified between the two treatment groups, comprising 376 up-regulated DEGs and 330 down-regulated DEGs. The identified DEGs were mainly associated with response to stimulus, biological regulation, and developmental process. RT-qPCR analysis was conducted on a subset of eight genes to corroborate the results of the RNA-seq data. The present study provides new information on the molecular response of biocontrol yeasts to heat stress.