Identification of ACC synthetase genes in Volvariella volvacea and analysis of their response to ethephon and 1-methylcyclopropene treatments
Juanqin Chen, Tianci Chen, Mingmeng Qiu, Ling Li, Qiangui Zhong, Qi Wei, Youjin Deng, Baogui Xie, Yuji Jiang and Bingzhi Chen
Scientia Horticulturae 278: 109848. (2021)
2021
บทคัดย่อ
Straw mushroom (Volvariella volvacea) is economically important in China, but after harvest, it can be stored for only 1–2 d, because it rapidly deteriorates. The role of the ethylene signal transduction pathway in the regulation of these processes in higher fungi is unclear. In this study, we aimed to identify and analyze the acs genes in the genome of V. volvacea. Bioinformatic analyses were performed to identify differences between the acs sequences of V. volvacea and homolog gene sequences of plants. Furthermore, ethylene production and acs expression in V. volvacea during storage were measured before and after ethephon and 1-methylcyclopropene (1-MCP) treatments. The results showed that there are four acs genes in the genome of V. volvacea, and the phylogenetic tree revealed that the proteins encoded by these genes had high homology to the ACS proteins in plants, with some differences. A conserved motif was found in these ACS proteins. Ethephon accelerated endogenous ethylene production and upregulated acs expression in V. volvacea, whereas 1-MCP inhibited endogenous ethylene production and downregulated acs expression in V. volvacea. Our study provides valuable data to further investigate the roles of acs in the mechanisms of senescence of V. volvacea. Further, acs could be used to develop molecular markers enabling screening and selective breeding of V. volvacea strains with slowed senescence, prolonging the shelf life of the product.